Applications for San Francisco (Batch 12) extended through September 15th, 2021!

Upside Foods

According to the IPCC, agricultural practices in all countries account for 18% of the total anthropogenic GHG emissions. The fecal run-off from animal agriculture pollutes our waterways, and puts the human race at risk with antibiotic-resistant superbugs. Replacing livestock would put a huge impact on our planet.

That’s why Upside Foods was the first to use cellular agriculture to grow the meat you love, in the lab instead of a farm. Upside was the poster child of cellular agriculture when it was hot in 2015, promising 90% carbon reduction compared to traditional factory farms. This is not a plant substitute; this is real meat. Smaller land footprint, fewer carbon emissions, none of the murder, and all of the flavor.

Geltor

Geltor is a conscious, biodesign company that produces advanced proteins sustainably and without animals, to service industries as diverse as beauty, nutrition, and food and beverage.

By combining precision fermentation and synthetic biology, Geltor is producing high quality, bioactive collagen and elastin products that don’t rely on animals for production, and don’t sacrifice on performance. Whether it’s a cosmetic or a food and beverage application – these high performance, advanced designer proteins are being efficiently produced by industrial microbes engineered by Geltor’s team.

Beeflow

70% of agricultural produce relies on bes for pollination. Yet modern agriculture has neglected pollination as a means to improving yields, and we know that bee colony collapse is a major risk factor for food security in the coming years.

Beeflow’s bee health supplements and bee hive management supercharge pollination, leading to an increase crop yields and improve the quality of fruits and seeds, without compromising the health of bees. By measuring pollination and bee-plant interactions Beeflow can help farmers plan water use, fertilizer use and enhance farm inventory management. The company has been running successfull pilot programs over the past two years, delivering unprecedented yields and now rapidly scaling to expand operations to farms across the West Coat and Latin America, covering almonds, berries and more.

NotCo

For decades, food R&D has been dedicated to make foods that are tasty, cheap, and profitable. In Matias Muchnik’s words, “that’s why the pinnacle of food R&D is a freaking Dorito.” That’s why the team at NotCo wanted to steer the next wave of food R&D to make it healthy, delicious, and affordable, focusing largely on plant-based foods. Why? First, it’s healthier and more sustainable. Second, Millennials’ preferences are driving this market and have already carved out $15 billion of growth.

NotCo is on a mission to take animals out of food production while not compromising on taste and nutrition. Their animal-free dairy and meat products are powered by Giuseppe – an AI platform with embedded food science to generate healthy and accessible products from plants. The company analyzes at a molecular level to indicate which plants must be combined in order to replicate their taste, texture, color and aroma, enabling manufacturers to discover a new source of fiber, calcium and proteins from the vegetables. NotCo delivers unprecedented experiences in milk, mayo, ice cream, plant-based meats; all tasting like the real thing.

Jungla Inc.

A huge problem in genetics is understanding what a particular mutation in a gene will do if it has not been observed before. BRCA1 is a well known mutation that causes susceptibility to breast cancer. We know that at the 185th nucleotide a deleted Adenine or Guanine can predispose one to breast cancer. This is because after sequencing thousands of breast cancer genes we observe this deletion event over and over and therefore have correlated the two events.

If if you sequenced your gene for breast cancer and they found a sequence that wasn’t normal but also not seen as cancer causing, what would you do? That sequence is called a Variant of Unknown Significance, or as is said in the industry, a VUS. While doing their PhDs and postdoctoral work at Stanford, Carlos and Alex had developed an approach to solve the mystery of VUSs. They used machine learning to look at the particular variant and predict its effect on the protein and the level of harmfulness of the variant. Later on, they developed a lab based assay to corroborate the interpretations, and further train the network (a critical piece of the puzzle we call “wet-in-the-loop”).

The largest genetic testing service to individuals and healthcare providers, Invitae, needed a solution to the VUS interpretation problem. With his skillset in hand, Carlos approached and closed, within four months, a pilot with Invitae to interpret VUSs in their samples. The pilot went so well that Invitae offered to acquire Jungla to incorporate their technology into Invitae’s offering.

STÄMM

Biology is the best platform for manufacturing cells. It can grow and produce all kinds of biomaterials very effectively. But for humans to be able to tap into this potential, we have to build bioreactors and figure out how to scale them in order to produce the massive amounts of biomaterials we need.

Bioreactors technology is very different when you’re optimizing for one reaction at one liter versus 100,000 liters, but this is where Stämm comes in. They are reinventing the way bioreactors work by developing a modular microfluidic platform that controls all the physical parameters, such as nutrients and dissolved oxygen, in a controlled environment. Unlike traditional bioreactor systems, their microfluidic bioreactor scales by adding more modules to increase the size of the reactor.

Sun Genomics

It all begins in the gut. We know that a balanced gut microbiome is key to a healthy (and happy) life. Probiotics, ingestible products that contain healthy bacteria and yeast, are available for people to buy and help with their digestive issues. But not all probiotics are created equal.

Sun Genomics found that an individual’s genome can help identify which probiotic strains are best for the person. By creating personalized probiotics, the company can eliminate the guesswork that’s involved with buying probiotics off the shelf and help people regain control of their health. Customers of all ages (babies, tots, adults) can get a whole genome sequencing of their gut microflora now, and get custom-tailored probiotics sent to their door right away, with the Floré product by Sun Genomics.

Sun Genomics has also launched a new academic collaboration focused on understanding the potential link between the gut microbiome and the Autism Spectrum Disorder

Prime Roots

Killing an animal and eating its flesh is not the only way to gain protein. Now more than ever, we need alternatives to conventional animal farming and fishing—not just because of animal welfare, but for human and environmental health. What’s happening now is not sustainable.

Prime Roots uses fungi as a complete protein source which acts as a seafood alternative. Described as healthful, protein-rich, sustainable, ethical, and delicious, the company is working to help people reimagine meat and seafood with fungi, that has the proper taste, texture, and nutrition. The product has already been tested with over 10,000 potential consumers, and their full product line is hitting markets and available for purchase on their website.

New Age Meats

Cell-based meats, or clean meats, can alleviate pressures on the environment, end animal suffering, and produce sustainable protein for the growing world population.

Yet, we are still far away from making cell-based meats a reality due to technological challenges. New Age Meats is tackling these challenges with automation and data to accelerate product development and bring pork sausages to market sooner. They are now developing a cell-cultured meat technology intended to offer vegan-friendly alternative meat products, by putting in hardware acquisition points to acquire more data and then assemble the data to make data-based decisions, faster, and with more insight. The fruits of their labor will be manifested in a delicious cell-cultured sausage coming to market soon.

New Culture

When we think of pizza, we think of gooey, melty, stretchy cheese. Unfortunately vegan cheese has not delivered. The texture is nowhere near as stretchy and doesn’t satisfy any foodie trying to replace their dairy cheese.

New Culture is making a meltable, stretchable cheese with the mouthfeel of cow-based mozzarella, by employing traditional cheesemaking techniques. In a manner similar to another key cheese-making ingredient, rennet, New Culture set about to eliminate cows from its cheese by wrangling microbial fermentation to produce caseins. These difficult-to-make proteins are the critical molecules that bring about that stretchy texture that makes cheese so cheesy.

Filtricine

Metabolism is what keeps us going. But for cancer cells that have abnormally high rates of growth, this is doubly true. The founders at Filtricine has found that cancers require key nutrients that healthy cells do not require.

Filtricine developed a targeted nutrient deprivation (TND) therapy for treating a broad spectrum of cancer. This enables doctors and medical care professionals to exploit metabolic dependencies to stop the spread of cancer without the use of drug substances. The product now a two step cancer therapy, the first step is a meal plan that excludes the key nutrients, which the Filtricine team has shown to slow down drugs in 4 different cancer types. The second step is a dialysis with a formulated fluid that removes the targeted nutrients from the body. They have already completed pre-clinical studies to get FDA approval.

NovoNutrients

As the world’s population continues to balloon, demand for seafood is going with it. Aquaculture is the primary method to meet demand, but relies on feeding billions of small fish to larger fish. A process that is inherently unsustainable and is only getting worse as ocean fish supply dwindles. C.E.O. David Tze started his journey investing in an aquaculture investment management company, when he saw the need for a growing food and protein supply.

NovoNutrients is looking to solve this problem with a radically different approach, growing high-quality bacterial protein from waste carbon dioxide. Their microbes break down industrial emissions of carbon dioxide and reassemble them into low-cost and nutrient-rich aquaculture feed and specialty ingredients, which sequesters the pollution and turns it into high-value proteins.

GateHouse Bio (fka sRNAlytics)

When your cells make instructions in the form of mRNAs, there are also many small RNAs (sRNAs) that regulate those instructions. They regulate the health and disease of all living things, and researchers are now finding out that their activity patterns are indicative of overall patterns in the body.

Turns out, this is a good strategy for biomarker discovery. Gatehouse developed an RNA analytics platform intended to classify patient samples with binary accuracy. The company’s platform identifies small RNA biomarkers and discovers molecular pathways of disease through deep analysis of small RNAs and accelerates the development of targeted therapies, enabling custom bio-markers and companion diagnostics driving cures.

A2A Pharmaceuticals

A2A is a partnership driven drug design company with candidates across cancer, infrectious disease, and rare diseases. Most of the pharmaceutical industry is focused on analysis of existing libraries of drug-like molecules with computational tools and high-throughput screening. By contrast, A2A is exploring 100s of millions of molecules in chemical space to develop proprietary databases of safe and effective new drug candidates. The approach enables rigorous pre-optimization of therapeutics before laboratory testing begins. Interest in A2As oncology programs with multiple pharmaceutical partner possibilities has picked up after revealing positive data from the Leukemia program.

They have a robust internal pipeline, with a focus on drug designs for breaking protein-protein interactions and collaborations with Daewood Pharmaceuticals, Novartis, and In Silico Medicine.

Amaryllis Nucleics, Inc.

Amaryllis Nucleics took a laborious and complicated chemical process to enable RNA sequencing and reduced it to a kit that non-experts can use and can complete the task in hours, when it used to take days. With a more accurate process, as well, Amaryllis Nucleics is democratizing access to RNA sequencing and in turn, acclerating the speed at which genomics discoveries are made by researchers.

Amino Labs

Amino Labs is pioneering accessible genetic engineering by enabling non-experts to begin learning and doing genetic engineering projects outside of traditional environments. With a technology ecosystem that includes a new type of lab equipment called a minilab, a wide range of genetic engineering wetware kits, and online software simulations, Amino Labs offers the most comprehensive range of genetic engineering education products on the market.

Amino Labs is unique in that it is the only company that tailors products for non-experts. Presently there are educational products that aid in teaching genetic engineering, however, they are designed and developed for “expert” teachers that have professional training (life science Ph.D or industry expertise), and for schools with traditional laboratory equipment. This represents a substantial barrier and we estimate that more than 90% of secondary schools, more than 47,000 schools in North America, are unable to offer genetic engineering education to their students.

AnimalBiome

AnimalBiome creates microbiome-based therapeutics to treat the more than 9 million cats and dogs in the US living with chronic digestive disorders. They have developed a patent pending process for creating a fecal microbiota transplant pill that is stable at room temperature and highly effective. Animal Microbiome has been treating cats and dogs for more than 3 years and over 90% have shown a decrease in the frequency of diarrhea, vomiting, and/or constipation. In the course of creating this product, they have also created the world’s largest database of microbiome samples in the order Carnivora, and are leverage this database to create next generation probiotics and veterinary diagnostics.

Ardra Bio

“Natural ingredients is the fastest growing segment in food, personal care, and health-&-wellness industry, but large companies can’t find reliable scalable supplies. For example, in 2016 there was an acute shortage of natural citrus ingredients because of citrus greening disease. Just the year before, prices of natural vanilla increased 10X due to low supply and increased demand.

Ardra’s synthetic biology platform unlocks multiple hard to make high value compounds through the fermentation of natural sugars, and their expertise in making designer microorganisms. Their platform utilizes computational methods in conjunction with a high throughput screening of proprietary enzyme libaries. This platform has already ben validated for multiple ingredients. Ardra has one joint development agreement to scale and distribute their first fragrance, leaf aldehyde. Deeper in their pipeline is animal-free Heme, Hazelnut flavor, a ketone mono-ester, cannabinoids, and several natural colors.

BioAesthetics

BioAesthetics’ Nipple-Areolar Complex Graft, or NACgraft, is a revolutionary next step in breast reconstruction that will provide patients who have had mastectomies with a new, natural, living nipple and areola. The NACgraft is a cell-free, biologically-derived nipple and areola graft that once applied by a surgeon, will allow the patient’s own body to integrate the NACgraft. This process uses the patient’s own natural ability to heal, resulting in a real and living nipple unique to and uniquely a part of the patient.

They are currently engaged in a clinical stufy for their NAC, and beginning development of enhanced biologically derived grafts — capable of sustained drug delivery — to improve outcomes for third degree burn survivors through an NIH grant.

Calyx

Traditional commercial gas sensors lack a middle ground: They are either really expensive and bulky, or they are small but lack sensitivity. There is a technical trilemma between specificity, sensitivity and, durability, and the devleopment of new chemistry-based sensors often have long development times and scalability problems.

Calyx uses a bio-based sensor that unlocks new opporutinies for indsutries that traditionally rely on chemistry-based sensors. Viruses are used to detect the analytes, and which changes the output of a colorimetric chip. This has high specificity, high sensitivity, and durability. Calyx’s platform has over a trillion receptors in one protein library, so with short development cycles, they can churn out new virus-based sensors for new customers in no time. Since 2018 their solutoins have been validated by major industry players, resulting in 5 paid pilots. Their go to market product will be targeting the poultry industry for ammonia monitoring and has already been demonstrated to outperform the state of the art.

Catalog Technologies

“The Cloud” is basically just someone else’s computer, taking up acres of land and consuming cities’ worth of power, and billions of dollars in cost of ownership. It generates more carbon every year than the entire airline industry. The amount of data we produce each year as a species is growing exponentially, but our storage capacity is not. Hard drives not getting smaller–we’ve reached the physical limits of that. There are also serious concerns about the archival materials in the long term. Hard drives need to be replaced every 5 years, and magnetic tape can maybe last 30 years.

Catalog Technologies might be the solution to that. Their writer can write over a terabit of data onto DNA in a single day. The system is basically a printer, but instead of ink, it’s DNA. Not only can this store DNA, but it opens up new possiblities through which we interact with archival data: If you have millions of copies of your information (as DNA) you can operate on all of them in massively parallel ways.

Circularis

Circularis’s technology screens a huge library of promoters in a single cell. Through directed evolution selection, the strongest promoters rise to the top and can be identified and incorporated into a host of gene expression applications, such as biomanufacturing and gene therapy. Beyond just promoters, their technology also works for enhancers, silencers/repressers, stability sequences, and nuclear delivery sequences. The process happens within days, instead of months.

Clara Foods

Clara Foods brews real animal protein without using a single animal — starting with the world’s first suite of animal-free egg proteins. Eggs the beloved breakfast staple has broad industry usage, delivering 20+ critical performance, flavor, and nutrition functionalities such as pH stability, tenderization and beyong enabling a $213B market in the foodand beverage industry. Their first product is an animal-free pepsin, which was launched in 2020, and is already selling across three continents. Clara has created a strong partnership ecosystem to truly compete with animal protein head on––a first in the alternative protein space––by partnering with ingredients giant, Ingredion (NYSE: INGR) for global distribution and ABInBev (NYSE: BUD), the world’s largest fermentation company, for production.

Dahlia Biosciences

Dahlia Biosciences is leading the development of cell-based RNA detection tools using CRISPR technology. The foundational RNA-targeting Cas9 technology was invented by Dr. Mitchell O’Connell and Dr. Sam Sternberg in the laboratory of Dr. Jennifer Doudna at UC Berkeley.

The company is dedicated to empower scientists to study and isolate cells at high resolution via sensitive, robust, and multiplexed RNA detection in both fixed and live cells. Their CRISPR based RNA detection reagents will plug seamlessly into existing flow cytometry workflows, reducing the time and cost-basis of research and development. Their kits and reagents can supercharge the many applicaitons based on fixed-cell analysis and live-cell sorting with wide-ranging implications for detection of disease to design of biomarkers and drugs.

DNALite

DNAlite Therapeutics is an early stage pharmaceutical platform developing nucleic acid medicines. mRNA has emerged as a leading therapeutic modality, but delivery remains to be a bottleneck. While current approaches inject mRNA into the bloodstream, DNAlite has created an oral approach to mRNA delivery that survives the stomach and gets taken up by epithelial cells in the gut. Once in the cells, the mRNA is read and the gut cells manufacture proteins — whatever is coded in the mRNA. These epithelial cells naturally turnover and refresh every 3 to 4 days, so DNAlite medicines could be taken on a regular cadence. The proteins expressed can be antibodies and other biologics; it can be equivalent to rare disease gene therapy, but essentially reversible, because no permanent changes are made.

The company has demonstrated proof of platform in-vivo, producing and quantifying proteins from orally given mRNA creating a completely new modality with broad and unique value. They are slated to enter the clinic with IL-22 as their first therapeutic target to tackle indications such as Inflammatory bowel disease (3 Mn
patients in US), Acute graft versus host disease of the gut (incidence of ~5000/yr), and Necrotizing enterocolitis (incidence: ~400/yr in the US).

Endless West

After a whiskey is fermented and distilled, it has to undergo an aging process, which is arguably the most expensive part of the process. The alcohol sits in contact with specially chosen wooden barrels whose wood contain compounds that are transferred to the whiskey, giving it exquisite flavor along with its color. Now imagine the same unique flavor profiles but for every spirit: wine, soju, sake, etc.

Endless West can simulate this process by analyzing the molecules that give spirits their flavors and aroma, and then synthesize their own “molecular” spirits, without the need for grapes, rice, or wood. Their first product, Glyph, is the first whiskey ever to be crafted ‘note by note’, using molecules (or, flavor and aroma notes) extracted in their purest form from more efficient sources in nature.

Finless Foods

If you’re eating fish, you might as well take a bite out of a plastic thermometer as a side dish. Studies point to significant amounts of mercury and microplastics found in nearly all the fish that we eat. Now is as good as time as ever to start using our technology to make better seafood, without being harmful to humans, nor harming any fish in the process.

Finless Foods started when the founders thought real hard about how our food systems are inefficient and environmentally devastating. They wanted to change this with lab grown fish: take a small sample of fish cells, and then grow thenm quickly and cheaply as a replacement for conventionally caught fish. To the consumer it will look like the fish they know and love to eat, but on the inside it will be very different. Finless’s fish is a return to the traditional fish that people used to eat before we polluted the oceans.

Girihlet

The complexity and growing prevalence of autoimmune disorders demands attention. Baked into our immune cells are records of the interplay between the genome, diet, and the environment.

Girihlet’s patented approach to sequencing T-cell receptors (TCR) unlocks these complex interactions by classifying TCRs into actionable biomarkers to diagnose and identify treatment avenues for autoimmune diseases.

Indee Labs

With their microvortex shedding technology, Indee Labs’ cell engineering platform preserves cell function while boasting high recovery efficiencies and each 5 x 10mm chip can process >50 million cells at >2-4 million cells per second and as low as 100uL in processing volume.

This matters to the population of patients who today cannot afford cell therapy due to the high cost associated with their production. With this system, the ability to engineer T-cells rapidly and more efficiently will make cell therapies more accesible.

Joywell Foods

Ten years ago, a founder at Joywell was researching ways to help his grandmother, a cancer patient, enjoy foods after chemotherapy wrecked her taste buds. He came across a molecule called miraculin, a protein that temporarily tricks your taste buds into thinking sour is actually sweet.

Over the last several years, people have been throwing “flavor tripping parties” that revolve around popping a tablet of miraculin and subsequently trying sour foods, only to then perceive them as sweet. Joywell took this idea turn make the future of artificial sweeteners, made not from carbs, but from proteins. Today, Joywell mass produces this protein using a bit of genetic engineering and microbial fermentation, they’ll be partnering with CPG companies to push out a wide variety of products to replace not only natural but artificial sugars. Watch out, stevia!

Mendel Health

True interoperability of the health care data system means not just one hospital sharing data with another, it means integrating all clinical trial notes and physician notes. Keyword tagging isn’t enough.

Mendel A.I. is the world’s best system for reading natural language — specific to healthcare. Their system turns unstructured data into structured data that can then be mined or searched and associated. One can imagine that what’s natural for a clinician to write varies highly by medical field and geography. Mendel is partnered with Efax — faxes are still the most common patient data transfer system in healthcare — to interpret instantly all medical faxes.

mFluiDX

Three years before COVID made everyone care about rapid molecular diagnostics, mFluiDx was already working on it. They were fighting epidemics before it was cool. Their initial prototype, funded by The Gates Foundation and DARPA, was working on rapid diagnostics for Zika virus. When patients would normally have to wait for days to get a result. mFluiDx’s prototype offered them an accurate diagnosis faster than a Spanish siesta, and 100X cheaper than a PCR setup.

mFluiDx presents a fast and portal DNA-based technology for identifyuing outbreaks of dangerous diseases in the field. Using microfluidics, they’ve engineered a field-ready, affordable, sensitive, and rapid approach that democratizes high-need diagnostics for everyone in the world.

mFluiDx selected as one of the top 20 startups to pitch at TechCrunch Startup Battlefield, from a pool of 900 startups globally.

MycoWorks

MycoWorks is a biotechnology company founded by artists with the mission to create a platform for the highest quality materials using Fine Mycelium . Drawing on over two decades of pioneering research and innovation by our creative founding team, MycoWorks’ proprietary Fine Mycelium platform has enabled a new class of premium, non-animal materials that are the next evolution in mycelium. The company mission is to using the tools of biotechnology, manufacturing excellence and creative, master craftsmanship to grow the future of materials.

MycoWorks is at the forefront of the movement to bring circularity and sustainability to the fashion industry. They recently annouced a landmark partnership with Hermès for a new material Sylvania, a hybrid of nature and biotechnology. Fine Mycelium™, the genesis of Sylvania, is produced in the MycoWorks facility. It is then tanned and finished in France by the Hermès tanners to further refine its strength and durability, and shaped into a luxury travel bag at the workshops by the Hermès craftspeople. The company’s flagship product Reishi™ — a non-animal, non-plastic leather that performs like cowhide — designed as a proprietary woven cellular microstructure inspired from animal leather. Reishi™ is available for sale for stakeholders across the textiles industry from running shoes to the runway.

NewWave Foods Inc.

Shrimp is the number one seafood consumed in the US and we import over $7 billion annually. But all of this comes with big health, social, and sustainability problems. Shrimp’s carbon footprint is nearly 10 times higher than that of beef’s. And it is very likely that you have eaten shrimp that has been caught and peeled with slave labor. No, not last decade–we’re talking last year.

New Wave Foods is bringing a sustainable shrimp to a plate near you. Using seaweed and other plant-based ingredients, their shrimp alternative has the taste and texture of real shrimp to any dish with shrimp served hot. They did this by analyzing the shrimp’s texture and elasticity at the molecular level to create the a high performance matrix. Together all this lets them create a superior product without compromising taste and nutrition.

OneSkin

OneSkin aims to promote skin rejuvenation by decoding the aging process. By replicating skin aging using 3D models and correlating biological markers with chronological age, they have created an efficient engine to screen new antiaging molecules. OneSkin is the first-in-class platform to quantitatively measure the rejuvenation effect on any molecule in human tissues

Their first topical supplement, OS-01, has been shown to act on a molecular level to reduce skin biological age, improve skin barrier, and improve cell function. OS-01 launched in Q1 2021.

Perennial

At all stages of our lives, our nutritional needs vary. Babies have different nutritional requirements, as do pregnant mothers and rapidly growing teens. Want to live forever? There’s probably an optimum diet for that as well.

That’s why Perennial is using their plant-based dairy platform to go after the elderly (>50) nutrition market with a high-value, indulgent drink, thoughtfully crafted to support the gut and the brains of aging adults. Perennial uses advanced technologies to take the best features of dairy and plant ingredients to break apart healthy fats and adhere them to the surface of plant proteins, making the resulting fat/protein matrix perform even better than actual dairy. Perennial is paving the way to purposeful nutrition that tastes good, and is easy to access.

Pheronym

We spend around $70 billion worldwide on toxic chemicals used as pesticides, but that number is shrinking, as places like Europe and even the US are calling on a ban on toxic pesticides that harm both bees and humans. There is still a growing need, however, for safe and effective pesticides to meet the world’s demand for food.

Pheronym’s technology improves plant health and crop yield using a new pheromone to control parasitic roundworms and control insect pests in the soil, enabling farmers and cultivators to apply bio-pesticides for eliminating insects without affecting the plant’s health and freshness.

Prellis Biologics

One of the biggest challenges of printing organs is replicating the tiny micro-vascularuture, which is nearly impossible to print with traditional 3D printers. No matter how advanced, you cannot cheat physics and fluid dynamics to quickly print something a nanometer thick.

So, to say that Prellis is like the laser printer of 3D printing is not so far off the mark: Instead of extruding bio ‘ink’ like traditional 3D printers do, Prellis invented a laser-assisted 3D organ printing system that can be controlled with light to build organs nanometers at a time (but scalably). They have the fastest high-resolution bioprinter in the world, 100 times faster than their nearest competitor, and their resolution is 10-100x better than any other bioprinting system.

They’re now creating living tissues in 3D to mimic biological functions that can be used in therapeutic development. In 2017, they injected their mini lymph nodes with Zika virus, and it subsequently produced antibodies for passive immunity against Zika. Their Externalized Immune System (EXIS) platform represents the first use of synthetic human tissue for de novo therapeutic antibody discovery.

Protera Biosciences

Consumers demand sustainable and healthier food ingredients to replace the chemical additives that we can’t even pronounce. Among these are the preservatives, texturizers, and emulsifiers that are buried deep in the ingredients label. The alternative is to find and produce proteins to replace these chemicals, but the bottleneck is the identification and design.

Protera Biosciences is advancing the science of protein engineering to address critical challenges in food, agriculture, the environment, and human health. They have a database containing billions of protein sequences from GRAS organisms, and screen them for the top variants, which are then produced in using yeast fermentation. Protera is optimizing the full protein engineering process, from design to testing and development of a proof of concept in less 3 months. Their antifungal protein (patent pending) has already been shown to prevent bread mold for more than a month, and are already scaling this up for commercial production.

Qidni Labs Inc.

We take our kidneys for granted. These organs remove waste, salts, and excess water, and we get to expel them from our body through urination. When you have kidney failure–like over 660,000 adults in the US–you become a dialysis patient, and have to drive into a clinic every other day to get hooked up to a gigantic machine that filters your blood for you. This can cost anywhere between $80,000 to $140,000 per patient per year. If only there was a way to have a portable, at-home kidney that you can take with you anywhere.

Qidni is making their own artificial kidneys, connected to the cloud, so they can enable independent dialysis providers to serve millions of patients without owning any clinics. Their QGel adsorbent can perform dialysis with 100X less water, and with significant cost savings. Qidni has already demonstrated the portability and viability of their artificial kidneys on a fully nephrectomized sheep (no kidneys). It survived, and the “kidneys” filtered out even more urea than is normally done in a typical dialysis session in a clinic.

QuantumCyte

When the team talked to oncologists about their pain points, they discovered that it all boils down to two things: slow time from biopsy to actionable result, and limited prognostic value because the data isn’t given them complete information. This isn’t surprising: 4-6 weeks to wait for biopsy results is a long time to wait for a cancer patient.

Quantumcyte can return information in less than a week and provide not just basic information to treat with standard of care, but other more important markers. The company returns 4x more data per sample because they have more precise methods and integrate AI into their system.
Their AI maps the tissue biopsy and identify specific regions (e.g., where the cancer cells are) to automate the analysis, thereby returning more precise data on significant portions of the biopsy, rather than losing it in the noise. Their AI gets better and better every sample it runs. This platform technology is extending beyond cancers, and currently testing on brain tissue and solid tissue (e.g., for psoriasis).

Sugarlogix

The profile of human breast milk contains human milk oligosaccharides (HMOs), which, over thousands of years, have evolved to carry special gut and overall health benefits just for developing babies. Not only that, but humans produce over 1000 times more milk oligosaccharides than other mammals.

Sugarlogix is creating those HMOs by yeast fermentation. HMOs are found naturally in human breast milk and is a key ingredient in feeding the good microbiome of the infant to cultivate a healthy gut and immune system. Using Baker’s yeast, Sugarlogix recreates these ingredients to add to infant formula, and other foods. Their goal is to create better nutrition for formula-fed babies, and then move on to the probiotic and functional food categories for adults.

SyntheX

SyntheX is expanding drug design into the ‘undruggable’ space for the treatment of cancer and rare diseases. They implement an innovative platform for the accelerated discovery of new classes of therapeutics that target the Achilles’ heel of cancer cells in a highly specific and selective manner. Traditional drugs work by disrupting the enzymatic function of proteins. SyntheX expands the drug discovery space from active site inhibitors to protein-protein interaction modulators using our innovative screening technology.

Ten63 Therapeutics

Using computers to design better drugs has been quite effective when there’s already a lot of knowledge about the compounds and the protein targets they’re hitting. But those approaches to in silico drug design haven’t worked well for new areas, where there isn’t a lot of knowledge. It’s the difference between interpolation and extrapolation.

To go after biological pathways where there hasn’t been much success in the past — and design entirely novel classes of drugs — a different approach was needed.

The Ten63 platform BEYOND, leverages a proprietary AI and state-of-the-art physical models to explore an unprecedentedly large chemical space, allowing them to discover new therapeutics to targets previously believed undruggable. While their competitors search through a few million compound variations for the best fit, Ten63 can easily and efficiently search through billions of possibilities. This allows them to also design drugs against all likely mutations of, for example, a tumor.

Mezomax

Non-union’ fractures are common in people with low and imbalanced hormones, such as the elderly, but may also result from infection, inadequate blood supply to the bone, and incorrect splinting. Surgery, which has a low level of success, is the current standard of care. Full recovery from non-union fracture surgery is rare and repeat surgeries are often required.

MezoMax created a first of its kind, convenient, twice daily oral formulation called MMX-701 that works by delivering calcium directly to the bone producing cells to accelerate healing and provide for more complete bone union in non-union fractures.

MMMX-701 was tested in over 1,000 patients in three international Investigators-Initiated Trials, using an open-label adaptive design, and has shown good safety and tolerability, a significant increase in the bone mineral density (assessed via Dual-energy X-ray absorptiometry as a T-score in the lumbar vertebrae), and decreased bone resorption.

Ember Medical Inc

Cardiac arrest is one of the most time critical conditions that can strike anywhere. For the patient, each minute until the ambulance arrives has a huge impact on survival and future medical complications.

Ember makes telehealth accessible in frontier markets and hard-to-reach regions around the globe. The company’s platform connects medical professionals via a video or a live chat to determine whether a visit to the physician is required, thereby empowering patients with the ease and accessibility of quality remote care while providing physicians with a full suite of virtual clinic tools.

CASPR Biotech

What do you do when all the Cas proteins get locked up in IP because of the CRISPR war? You go to the ends of the earth to find an undiscovered Cas protein. And that’s what the Caspr team did, traveling to high-altitude mountains and volcanoes to find extremophiles in Argentina where there might be a Cas protein whose upbringing might endow them with temperature resistance.

Caspr has been acquired by a Fortune 100 company.
Now Caspr is using their special Cas to develop cheap and rapid CRISPR-based test kits ƒor infectious diseases. One month after they graduated from IndieBio, COVID-19 hit and Caspr’s team knew exactly what they had to do. They flew out their scientists out of Argentina to come live in San Francisco, where 15 of them lived in a single house in Alamo Square, all trying to develop tests for the coronavirus.

After demonstrating how rapidly they were able to develop their tests, Caspr was approached by Amazon to help with their internal efforts to test their employees.

Serenity Bioworks

Serenity is a therapeitucs company focusing on cellular stress pathways. Their premise is that when cells encounter a stressful environment — whether it be a lack of oxygen, increase in temperature, or exposure to a toxin — they express a myriad of stress-induced proteins, like heat shock proteins (HSPs), to maintain the cell’s order. In some instances, cells also secrete and export these signals to communicate with immune cells to avoid cellular destruction and inflammation, and maintain self-antigen tolerance. The benefits of exercise, sauna-use, and fevers, have all pointed towards stress-induced signals that can be used to treat diseases. Loaded with this insight and leveraging recent academic research and tools in stress, genomics, proteomics, and systems immunology Serenity is developing a pipeline of new medicines that will address urgent unmet needs for autoimmune disorders. They call their platform StressTek™ — enabling therapeutic targets from endogenous cellular stress pathways.

Their lead candidate SER-101 is targeting Lupus through this platform. The company will continue to explore the interface of stress and immunology to develop the next generation of StressTek™ therapeutics.Serenity’s lead therapeutic for Lupus, SER-101, is shown to preserve kidney function in a lupus mouse model and was demonstrated as safe in a phase I/IIa clinical trial.

Oralta

Most tools for oral health treat symptoms, rather than the root cause of disease, dysbiosis of the oral biome.

Oralta is solving this by blending highly effective strains of probiotics to restore a healthy equilibrium of beneficial bacteria to outcompete odor and disease causing bacteria. After selling out of their first batches, they are now scaling product to meet consumer and dentist demand. Long term they are building a database of strains based on high through out testing of strains in combinations to treat consumer and clinical oral health conditions.

Nuro Corp

With the 17 million new patients every year who are recovering from a stroke, a third of them will be touchless and voiceless. These victims have aphasia and paralysis to a point that they can no longer interact with the rest of the world, and can bring an individual to a such an imprisonment in their own bodies, that life feels like it no longer matters.

Nuro is making a headset and multimodal, multidirectional operating system (dubbed NUOS) to help a locked-in patients communicate with the world. Their headset is combining EEG and EOG into a set of algorithms that give stroke patients their independence and dignity back. You can view their current work on their website where they show patients communicating independently with just their brain – no voice, no touch. They are the only manufacturer in the world cleared by Health Canada, US IRB and US FDA under ‘Expanded Access’ for Communication by Brain for up to Locked-In Stage VI ALS.  NURO has developed a complete neurological product line named NUOS, which can be implemented instantly without any brain surgery and with a wide range of individuals, from healthy human beings to victims of Stroke, Trauma or Neurodegeneration.

Neurocarrus Inc

Traditionally, people in pain are given Opioids—which, although they relieve pain, can have dangerous side effects such as loss of motor function, and addiction. Ocycontin, Vicodin, and other pain relievers are effective in subduing pain, but they are extremely addictive, which causes major problems in families and communities.

The Opioid Crisis is a reality we’re facing now—but what if pain could be treated another way? That’s exactly what Neurocarrus has been working on. Their new drug delivery technology safely disrupts pain signals without possessing addiction-forming qualities. The company’s lead asset, N-001, is a novel protein that was synthesized to target key sensory neuron structures called actin. It is designed to disrupt peripheral pain signaling without affecting motor function or causing addiction.

Michroma

The vast majority of food dyes used today are synthetic chemicals derived from petroleum which have been linked to allergies, hyperactivity, and even cancer. Biocolorants do exist, but they are expensive to use and don’t hold their color when cooked or processed; insect-based dyes aren’t halal, vegan or kosher.

Michroma’s sustainable solution uses edible fungi cells that express the natural colorant into the bioreactor. No solvents are needed to extract and purify the color. The performance characteristics of their dyes are far superior to existing biocolorants.

Membio

Membio is developing a world class biomanufacturing platform to enable the cost-effective and scalable production of whole cells, including red blood cells. Blood is a necessary prerequisite for many life-saving interventions, making a reliable blood supply an essential part of any healthcare system. Membio is on a mission to provide the world with a safe and sustainable supply of blood.

Lingrove

Wood is a touchy subject. It could be considered a more sustainable material than plastic because it’s regenerative, but we’re simply just not regenerating them fast enough.

Lingrove is a materials company that is making wood without the trees. Their signature material, Ekoa, is lighter than carbon fiber, stiffer than fiberglass, and the wood grain finish is simply beautiful. Their product is made from a bioresin bonded with flax, which is normally discarded anyway after they are harvested for flaxseed. Ekoa can be molded into complex shapes and dyed to match the finishes of even the most expensive hardwoods. High performance, planet-positive and carbon-negative products without compromising the beauty.

Intrinsic Medicine (fka Lupa Bio)

Modern diseases are a result of cultural evoluton outpacing biology, and so inflammation has become maladaptive. The safety issues limit thet current repertoire of anti-inflammatory drugs from serving a majority of patients. In 2018, human milk oligosaccharides (HMOs) were discovered in the blood of pregnant women and an epidemiological study showed that elevated levels of key HMOs were associated with rheumatoid arthritis remission. Out of the 600 quintillion potential glycans, 300 million years of mammalian evolution have sorted out 200+ HMOs that are functionally bioactive in the body.

Intrinsic Medicine is a platform company innovative safe, bioactive sugars (like those HMOs) as a new therapeutic modality. With a combined 30 years of pharmaceutical industry experience at Ionis, Jason and Alex have been are using two key HMOs to be the only safe and effective solution for young people with irritable bowel syndrome, and they are taking their lead assets to clinical trial now.

Dalton Bioanalytics

Dalton Bioanalytics has developed a new way of analyzing all of the different types of biochemicals in blood. Using tried-and-true liquid chromatography mass spectrometry, they measure proteins, lipids, electrolytes, metabolites, and other small molecules–providing a more complete analysis at a fraction of the cost while maintaining data integration and integrity.

This data rich platform has applications for human health, from discovering novel biomarkers to developing new clinical tests to drug development. Our vision is to create a single low cost liquid biopsy that can be used for early detection across a wide range of common and rare diseases and conditions. Dalton is also working with many Fortune 500 companies to understand and develop better food and consumer goods, and optimize bioprocesses for industry.

Convalesce Inc

Convalesce is a biotech company pioneering local in situ genetic and cellular reprogramming, such as turning glial cells to neurons, or alpha to beta cells to replenish insulin. Reprogramming these cells ex-vivo has compromising limitations and nucleic acid medicines can’t effectively localize in vivo.

Convalesce have developed a platform that enables hyperlocal cell reprogramming through advanced materials harboring genes and/or cells of interest in vivo. We have demonstrated that CNV platform’s ECM can provide robust cell survival (3X) in vivo and enhance targeted differentiation. We were also able to get 2.5X higher expression of a gene-of-interest. Convalesce is leveraging its platform initially to target eye disease, dry age related macular degeneration (AMD) and is raising series seed to complete pre-clinical development by 2022.

Chronus Health

Every day blood panels are one of the simplest tests that are foundational to healthcare, but patients are still waiting days to receive their lab results. This results in multiple unnecessary visits to the doctor, and delayed time to action. Imagine what we can do if we were to get near-instantaneous results on our blood panels.

Chronus Health’s portable device allows for real-time diagnosis at the point-of-care providing lab results in minutes, dramatically improving time to care and patient outcomes. Their initial launch for Complete Blood Count (CBC) and Complete Metabolic Panel (CMP) accounts for 50% of all blood tests performed.

The prevalent techniques for doing blood test relies of optics and use of mechanical pumps for fluid preparation–this drives up both the size and cost of the devices.Chronus uses electrical methods for sensing and sample preparation that reduces the device size and cost.

Starting with CBC and metabolic panels, Chronus has already demonstrated on-demand blood tests that meet CLIA standards.

Biolumen

Every Biolumen fiber pill is as effective as 21 conventional fiber pills. Taken with meals or snacks, Biolumen knocks 15-20 points off your sugar spike, reducing insulin response.

Working with ProDigest’s artificial gut system, the gold standard in microbiome assays, SugarSink Superfiber showed dramatic benefits:
+37% butyrate
+34% propionate
+37% acetates
Biolumen is working with two international corporates on product lines of snacks.

Vertical Oceans

90% of the fish consumed in the U.S. comes from China and overseas

Vertical farms are exploding, but why grow lettuce? There’s something else you can grow that generates 12x more revenue, on the same minimal land footprint. No, it’s not marijuana. It’s shrimp – in multi-story vertical oceans.

Humankind’s future will include cultured meat and plant-based meat, but it will also include sustainably grown animal protein. What “self-driving” is to the car industry, “self-cleaning” is to the booming aquaculture industry. Zero waste, zero effluent. With Feed-Conversion-Ratios (FCRs) below 1.0. Vertical Oceans aims to be the world’s first seafood company to reach these hallowed benchmarks. The whole system is run on their biocomputer. Having just moved from their pilot facility to a larger demo facility in Singapore, they’ll be running four harvests this spring and are coming to Chicago soon.

Sundial Foods

The AltProtein space is abuzz, but lacking in products that engender the same finger-licking scrumptiousness of devouring a plate of tasty wings. Sundial Foods developed a novel processing technology for creating plant-based whole cuts of meat — complete with skin, meat and bone. The beauty is in how it ends up on your plate from only eight clean-label ingredients.

Before they were even in our program they were already selling their 1.0 product in the grocery stores in Switzerland. During the IndieBio program, Sundial has increased their degree of automation from 30% to over 70%, and acquired key new hires to help push them towards bigger production. By the end of 2021, they’ll be starting at the Rutgers Food Innovation Center, where Impossible Foods also got their start.

Spintex

Spider silk is renown for being one of the strongest materials on the planet. Light as a feather but stronger than steel (by weight). Scientists have been to make artificial silk for a long time, but have failed to produce them at scale and in industrially amenable conditions.

Spintex has invented an entirely new, scalable method of making silk, using a naturally derived protein. Their critical insight is that, when it comes to spiders, how they spin fibers is as critical, or even more so, as the proteins they use. By mimicking spider spinning, and using a natural protein, Spintex is already surpassing premium silk in performance, with no compromise on look and feel. They’re starting with the textiles industry and will eventually move towards high-value medical applications.

Sequential Skin

Sequential Skin provides the world’s first truly personalized skincare solution. Their easy-to-use at-home test kit uses advanced genetic sequencing technology to understand how your genes, skin microbiome, and environment interact to define the unique needs of your skin. They then match you to tailored skin care products that provide the exact ingredients that will work best for your skin, to boost your overall health.

Reazent

Plants have evolved to live in a symbiotic relationship with microorganisms such as bacteria and fungi present in the soil. As one would expect, soil bacteria play an outsized role in plant growth, but making it a product that the agricultural industry can use en masse has been difficult.

Reazent makes truly biological fertilizers by leveraging this symbiotic relationship between plants and bacteria. By being able to turn on a metabolic switch in bacteria, Reazent can make a fertilizer that is organic and more sustainable than the leading agrochemicals, while also being as (or more) effective. Reazent’s biological fertilizer can be applied to any crop, and is currently being tested in multiple field trials all over the world, and has already acquired a scale up partnership.

Proteinea

The major lesson of the Cleantech 1.0 era was “reduce or avoid the Capex.” Today, in the new Cleantech 2.0 era, we are always looking for companies that can scale up without the huge investment needed into expensive facilities.

Proteinea is a new approach to the field of precision fermentation. You might call it “bioreactor-free.” Rather than growing high-value complex biologics in e coli, or in yeast, or even in insect cells – inside large steel bioreactors – Proteinea uses whole insect larva, growing on low-cost feedstocks. These larva are inoculated and produce the target biologic in every cell, then through downstream processing, the target biologic is extracted and purified. 

With this approach, bioproduction can scale up far more rapidly and with ultra-low capex spend. Right now, Proteinea is working on multiple projects with three corporate partnerships. One is scaling up a polio-vaccine. One is an aquafeed vaccine. And one is a biologic drug. 

Pando Nutrition Inc.

Antibiotics are like nuclear bombs for the microbiome, completely wiping out the bacterial community to promote livestock growth. But what if we can nurture the animal by selectively promoting good microbial consortia while eliminating the bad ones? And what if we can save the planet in the process?

Pando Nutrition is leveraging their designer probiotic as a replacement for antibiotics, and has shown to outperform bacitracin as an antibiotic, while also increasing chicken growth by 10% and making them healthier. Their product has also been shown to reduce methanogenic bacteria in cows, which produces the potent greenhouse gas, methane (which is why their burps are so bad for the planet!). The ultimate goal is to completely eliminate methane-emissions from cows, to create carbon neutral beef.

Ozone Bio

Can Green Chemistry be a high-margin business? The conventional wisdom has been “no.” Bio-Nylon, for example, has a big Green Premium because manufacture is so expensive. Ozone Bio changes the bio-nylon industry’s economics — enabling high margin and massive growth. In addition, they can make Nylon a circular-economy product for the first time, using lignin waste from the paper industry. Their process is so super-scalable that they are already working at 50 liters volume.

OzoneBio is producing an emissions-free, low-cost adipic acid produced from waste feedstocks to make the world’s only bio-nylon 6, 6 that can compete in the open market. No green premiums needed here. The zombie cell technique invented by co-founders Khorcheska Batyrova and Anna Khusnutdinova eliminates the need for costly metal catalysts. This adipic acid is scalable, cost competitive with petroleum-derived products, and can be made from wood-derived aromatics.

OncoPrecision

OncoPrecision can predict a patient’s response to cancer therapies by replicating the tumor microenvironment ex vivo. Their unique, triple co-culture method increases patient-derived cancer cell viability 60x in a 2.5D format that has been validated using real patient data. For patients in dire need of the right cancer treatment the first time, OncoPrecision offers doctors the assurance that decisions made today will make the difference tomorrow.

When time is of the essence, being able to screen potential therapies with your personalized cancer avatar in under 10 days is fast. Doing so with more accuracy than today’s sequencing or organoid approaches is huge. Through their growing list of strategic partnerships, OncoPrecision is screening, selecting, and speeding up the delivery of precision oncology.

Nyoka Design Labs

Nyoka Design Labs is creating an ecologically prosperous future by developing earth-friendly technology to replace standard toxic, wasteful products. They use sustainable biotechnology and material science advances to build inspiring & innovative tech following a circular economy model.

Nyoka is tackling the $4B portable lighting industry by developing two deeply sustainable technologies designed to replace single-use glow sticks and LEDs while simultaneously innovating the packaging required to successfully deploy these technologies for recreational, industrial, and humanitarian purposes.

Multus Media

Multus Media develops the key ingredient to make cultivated meat affordable and profitable. Livestock agriculture contributes to 15% of all greenhouse gas emission globally which is more than the entire transportation sector combined. Cultivated meat brings an 87% reduction in CO2 equivalent emissions, along with 96% less freshwater use and 99% less land use to produce the same amount of meat. ​

This is why our mission is to design growth media that provides a collective benefit to the whole industry by lowering entry barriers, shortening routes to market, and supporting scale. Multus Media is accelerating the forefront of an industry that will replace the devastating impacts of livestock agriculture.

microTERRA

microTERRA has developed a unique functional ingredient for the plant-based food industry, which has no color or taste, enabling customers to deliver nutritional value without sacrificing the texture, taste, or presentation of plant-based products that aim to mirror traditional protein sources.

microTERRA partners with existing aquaculture farms in Mexico to grow lemna, an aquatic plant, in a scalable, affordable, and sustainable way. microTERRA is able to leverage these aquafarms to produce lemna 3-5 times cheaper than competitors, while providing farmers with an extra revenue stream, cleaner aquafarm water, and a reduced water footprint by two thirds. microTERRA’s vision is to create ingredients that instead of using water, are cleaning water.

Microgenesis

Microgenesis has worked with some of the hardest infertility cases there are – women who have undergone and failed multiple rounds of IVF. With a cohort of 287 would-be mothers, over 75% of their patients became pregnant using their proprietary test and nutraceutical regimen. Microgenesis uses microRNA signatures, on swabs from the fertility biome, to diagnose 64 phenotypes. They treat patients with 53 combinations of probiotics, nutraceuticals and diet-change. With personalized nutraceuticals and probiotics it resets fertility in 90 days.

Lypid

We’ve had an on again, off again relationship with fats. One crucial challenge for plant-based foods is that they’re missing the fat. Most of the time, they incorporate coconut oil as an ersatz lard or butter, and it’s simply not hitting the spot. As a B2B company, Lypid is putting a new spin on fats to bring lip-smacking goodness to the exploding plant-based meat category.

Despite huge sales jumps, plant meat is only 2.5% of the protein market. For the next wave of growth, plant meat needs to be more like animal meat.

Vegan oils have different textures, melting points, water content, and flavor-volatility than animal fats. Lypid are the inventors of PhytoFat, a line of fats made from vegan oils that can be tuned to precise textures and melting points. They do this without additives like texturizers, emulsifiers, or hydrogenation. Their secret? Water. Which makes their products healthier, lower cost, and more fat-like.

Liberum Bio

Today, to produce any sort of custom recombinant protein, it takes weeks. This dramatically slows down scientists’ ability to test, iterate, and improve. Liberum’s affordable, benchtop protein printer will cut that time down to a few hours.

Kraken Sense

Kraken Sense is bringing real-time testing, with results in under five minutes, to large-scale food and water systems. Deployable throughout the system — farms, processors, kitchens — their technology blunts the toll that food recalls take by eliminating the time delays in food safety testing. Their in-line autonomous device uses carbon nanotube-based, single-use cartridges to perform strain-specific pathogen detection and quantitation on-site.

Jointechlabs, Inc.

Jointechlabs is a regenerative medicine solutions company based in California providing FDA-cleared technology that empowers safe, cost-effective point-of-care regenerative medicine therapies to eliminate pain and suffering, and make you look and feel better. With 9 granted patents, signed distributors, efficient team, NIH grant, early sales, and several pipeline products, Jointechlabs is creating a regenerative medicine ecosystem as a standard of care.

Their device, MiniTC, has been shown to isolate fat-derived stem cells at in a disposable, closed loop medical device in any clinic setting, with no change in infrastructure. It’s fast gaining adoption in medical aesthetics, plastic and reconstructive surgeries, orthopedics and wound care. This platform is unlocking a therapeutic development program, their first program in clinical development, JTL-T-01, is a proprietary injectable stem cell biologic therapy for osteoarthritis, and is already approved under FDA’s 510k program.

Khepra

With our current technologies, the world’s global production and supply of energy will not be able to meet future demands. This is doubly true as more and more corporate promises are flooding in, aiming to achieve “net zero” by 2050. We need new technologies not only to produce and store energy, but also to move us away from carbon-intensive sources like petroleum.

To meet the diverse needs of a global energy market, Khepra uses fuel production as power storage for excess renewable electricity. Khepra is building reactors that deploy high intensity ultrasound frequencies to take flaked waste — everything from unrecyclable plastics to biomass to cardboard — and even mixed waste — and break the chemical bonds in the waste. This releases valuable chemicals and fuels, which are readily sold to refineries. Zero waste remains — everything in the reactor becomes fuel. Khepra will be working with Alabama Energy Group to upcycle their pulp and paper waste streams into fuels.

Ivy Natal

Ivy Natal is developing a process to create healthy human egg cells from skin cells as a treatment for most cases of infertility. Ivy Natal is developing a novel process to create healthy human egg cells from skin cells, giving women the confidence in their ability to choose whether and when to have a child. Many fertility patients cannot have children except through the use of donor eggs. This can be due to surgery, chemotherapy, maternal age, or genetic conditions. Ivy Natal aims to enable these parents to have genetic children for the first time.

Innate Biology

Dr. Chris Rhodes, the CEO of Innate Biology and nutrition influencer with over 150K followers on TikTok (@thatnutritiondr), wants create products that can support the massive intermittent fasting industry. Innate Biology has invented the world’s first “fasting mimetic” supplement designed from years of human research at UC Davis to give people the benefits of fasting without the fasting. How? By supplementing with a combination of the same beneficial bioactive metabolites your body naturally produces during a prolonged fast.

Unlike their competition, Innate’s fasting mimetic is designed from human research, for maximum human benefit. These are the compounds that evolved in your own body over millennia to induce a “bio-program” of cellular health, regeneration, and longevity. The results? Innate has shown that their fasting mimetic extends lifespan by a stunning 74% in model organisms and creates powerful anti-inflammatory and antioxidant effects in human cells.

Huue

Jeans are everywhere. But the blue dye in most jeans are made with chemically synthesized indigo, which not only pollutes our planet (both chemical and and carbon), but the entire process is also toxic, corrosive and highly combustible. We don’t have to deal with these issues when we buy them at the store, but someone (the person who makes your jeans) does.

Huue discovered a way to fix these problems by using a nature-inspired process to create indigo (and other colors) from completely renewable sources like sugar and microbes. They’ve programmed bacteria to help make the most sustainable jeans on the planet, and proven that their dyes perform just as well as the toxic ones. Aheir color platform isn’t stopping at denim–they’ve already moved on to food dyes, and is partnering with Gingko Bioworks to expand even further.

Harmony Baby Nutrition

One in six babies suffer allergies to formula, leading to painful cries and sleepless nights for mother and infant alike. Alternatives such as soy-based formula or hydrolyzed cow’s milk formula are expensive and can also have violent side effects like diarrhea and vomiting.

Harmony Baby Nutrition is a food science-based company that uses precision fermentation to produce human breast milk proteins and create infant formulas that most closely mirror human breast milk. Harmony offers the only dairy-free, allergy-free, and environmentally friendly baby formula in the world.

Halomine

15 minutes after cleaning a surface with bleach, the bleach disappears, and a surface can be recolonized by nearby microorganisms. Now ask yourself: how often are surfaces cleaned with disinfectants?

Halomine’s technology acts as a bleach extending agent. Their first product, HaloFilm™, is a simple spray that creates a thin-layer film to hold bleach molecules close to the surface. The film is safe to human touch and can be “recharged” with fresh bleach applications to maintain disinfection. HaloFilm™ extends surface disinfection against fungi, bacteria and viruses, including SARS-CoV-2.

Free to Feed

Free to Feed is dedicated to finding solutions for mothers of food intolerant infants. The Free to Feed allergen test that identifies various food proteins in breast milk, allowing breastfeeding mothers the opportunity to determine what is eliciting a response in the infant. 

Free to Feed aims to offer women a solution other than blindly trialing elimination diets or switching to hypoallergenic formula. Their product will be an alternative and complementary option for mothers to understand what allergens may be present in their breast milk at the time of feeding.

Electro-Active Technologies

We have two major problems plaguing humanity: there are food shortages all around the world, AND we need to increase the proportion of renewable energy and capacity for energy storage. What if we can solve both these challenges, feeding two birds with one scone? 40% of our food is wasted and accounts for a sizeable proportion of methane emissions, a potent greenhouse gas, while it’s in landfill.

Electro-Active Technologies diverts this food waste into renewable hydrogen using their modular reactor. The liquid fraction of food is converted into hydrogen, while the residual solids can be composted or turned into an animal feed, all with the power of microbial electrolysis. In 2021, they they have a $1M partnership with Southern Company Gas to make distributed, sustainable waste-to-value solutions a reality.

Cybele Microbiome

The beauty industry will tell you: your skin is finnicky. That’s why there are over 1000 products for different face types and beauty enthusiasts can tell you what a pain it is to find the right one for your particular face. Each of our skin biomes have preferences from pH and moisture, to even the balance of bacterial consortia.

Cybele (pronounced SIB-uh-LEE) thinks of cosmetics products not as a mask, but rather as FOOD for your skin biome. They don’t try to drown your skin in new microbes that don’t belong–instead, they feed your skin bacteria to control what they do and produce. One of the products that your skin biome can produce on its own is ceramides, touted in the industry to retain skin moisture. By feeding them PRE-biotics your skin can produce the high-value probiotics you want, in the balance made for your skin.

Chi Botanic

The most valuable plant products are difficult to grow, and make up a $250B industry for fragrance and flavors. Here’s the kicker: the most valuable parts are only a TINY fraction of the overall plant. So, why grow the entire plant just to get the small amount of the most valuble parts?

Chi Botanic overturns 10,000 years of agriculture, by not growing the whole plant. Instead, they take the source plant, convert it to stem cells, and then grow plant cell cultures, which grow 30 times faster than a normal plant, and requires neither the land area nor the pesticides and fertilizer to grow it. Their go to market product is aloe, and then moving towards other plant cells, like raspberry ketones which cost $20,000/kg. Chi Botanic is not just a plant product company–their plant cell culture is a full platform with high throughput robotics, proprietary cell lines, and genetics know-how.

Diadem Biotherapeutics

One way cells communicate to each other through long distances is via exosomes. Originally thought to be cellular garbage, exosomes are now a powerful opportunity for therapeutics. Many competitors are focused on packaging inside the exosome to use as delivery vesicles, and thus completely ignores the shell as a modality for therapeutics.

Diadem Biotherapeutics is engineering a platform to decorate the outside of exosomes, so that they act as signaling molecules. By engineering the surface of exosomes, with signaling agonists, they can craete a new therapeutic modality, 10,000X more potent than the unattached protein. Moreover, they can engineer potent combinations of multiple receptor agonists. The team is currently performing IND-enabling studies for oncology and autoimmune disorders.

Diptera.ai

Mosquito-borne diseases like dengue, Zika and malaria infect hundreds of millions every year. Global warming is driving these mosquitoes to spread rapidly, endangering many more.

Diptera.ai’s technology cuts the cost of sterile insect control (SIT) – a natural and highly efficient insect birth control method – to make it a widespread solution against mosquitoes. Diptera.ai combines computer vision and deep biological knowledge to fight mosquitoes and their diseases. Diptera.ai uses non-GMO technology that eliminates the need to build a mosquito rearing and sorting facility near every release site.

Cayuga Biotech

Serious bleeding is a major cause of morbidity and mortality worldwide and outcomes are linked to how quickly hemostasis can be achieved. Excessive bleeding is a global threat that can affect all populations, but is especially critical for patients on anticoagulant therapy or those who have a rare bleeding disorder. Cayuga Biotech’s mission is to harness the potential of polyphosphate to address uncontrolled bleeding episodes.

CAY001 is a first-in-class drug which aims to accelerate the body’s natural clotting response even in patients with compromised platelet function. Unlike natural platelets, CAY001 is can be mass-manufactured, is stable at room temperature, and does not require type matching, allowing it to be deployed with first responders.

Carbix

Concrete is one of the oldest forms of building materials, made of limestone aggregates and the cement that binds it. But making the limestone aggregates involves a chemical reaction takes millions of years to do naturally. Carbix has created a reactor that can do this millions of times faster, all while taking emitted carbon and embedding it into the concrete.

This is local, sustainable, carbon-neutral concrete. Their reactor can embed 20X more time CO2 than most of the competitors out there. For every metric ton of concrete produced, more than half of it comprises of sequestered carbon. A single reactor with a 1 cubic meter array can clean up an entire neighborhood of energy production, and produce enough concrete for the foundation of 500 single family homes. With Carbix, all concrete can be carbon-neutral.

Capra Biosciences

In fermentation, biologists focus on free-floating bacteria and yeast for making products today – and if biofilms develop, it’s a big problem.

Capra reverses that equation. In nature, 99% of all bacteria exist in the biofilm state, and at Capra, biofilms are the solution. Biofilms mean more cells packed tightly together, working together as a team. Capra designed their unique bioreactor around this unique insight, resulting resulting in a process that requires less infrastructure, up to 100X water, and minimal downstream processing. These bring cost savings up to 90% on CAPEX and OPEX.

Capra is making high performance lubricants for the $150 billion motor oil market. But first they’re making a bio-Retinol for the cosmetics market.

Canaery

Canaery will be a security company, detecting and identifying dangers in all goods and people – at ports, airports, inspection points, and borders. They don’t use X-Rays, nor do they use cameras. Instead, they will use scent. Absolutely everything in the world gives off a unique signature of volatile compounds, perpetually – a scent-fingerprint.

Being able to detect and identify everything through scent has long been a holy grail in tech. Canaery is a neural interface company from the Rinberg Lab at NYU, the world’s leading lab for how olfactory information is represented in the brain. It can detect critical information for security to health and from farms to ships. Canaery will do for scent what machine vision has done for sight.

California Cultured

Around a decade ago, cocoa bean cells were cultured in giant, 20,000 liter bioreactors – it worked, except for one little problem. To get the cells to grow, they had to use the main toxic ingredient in the herbicide RoundUp.

California Cultured has solved that issue by enticing cocoa cells to grow with stimulation by food ingredients you’d find in any grocery store. Second-time founder Alan Perlstein (Joywell Foods) leads this team, which is going to market rapidly with a D2C subscription model.

The benefits extend beyond social impact. California Cultured’s cocoa cells are less bitter, which helps human health. This is important because conventional chocolate can be 50% sugar to counteract cocoa’s bitterness. California Cultured is aiming to reduce sugar content 10x.

Bucha Bio

Over 3,000 years old, leather is a part of humanity’s shared heritage and the material of choice for creators around the world. But the irreversible damage of the animal and plastic leather industries is unacceptably unsustainable, driving the world’s leading brands and creators into action on a search for a new sustainable solution. Bucha Bio materials are entirely animal & plastic-free while preserving the characteristics that creators love about leather: a classic texture, high durability, and boundless design potential.

Brightcure Ltd.

BrightCure’s bioactive ingredient continuously protects, restores and balances your intimate skin. Our products calm your urogenital area which can be irritated and damaged by antibiotic therapies and harmful bacteria living in your gut and colonizing women’s intimate area. Unhealthy food, irritation, and changes in pH lead to reduced urogenital comfort making it easier for bad bacteria to grow on your skin.

BrightCure’s ACTIVE COMPONENT kills the harmful bacteria and activates the skin’s natural ability to repair and protect itself, thereby boosting your natural immune system to promote a healthy woman’s intimate microbiome.

Blue Planet Ecosystems

Aquaculture has its problems, with the majority of the fish being fed fishmeal and a constant stream of antibiotics, with all the waste effluent going back into the ocean, affecting the already ailing ocean ecosystem. What if we can recreate the ocean in a tank, decoupling it from our natural ecosystem, so that seafood can be generated just from the sun?

Blue Planet Ecosystems miniaturizes an ecosystem in a closed-loop, recirculating aquaculture system facilitated by computer vision, to turn sunlight into seafood. The sun feeds the algae, which feeds the zooplankton, which feeds the fish (no need for fish meal or pesticides!). Quality is controlled by computer vision, which monitors fish health. Best of all, this can be done on land, in the desert, without needing an entire ocean at your disposal.

BioROSA Technologies Inc.

No blood test exist to confirm autism spectrum disorders (ASD). Wait times for diagnosis range from 3-24 months, and the average age of diagnosis is around 4.5 years, when it’s confirmed by behavioral symptoms. More than 60% of autism health-related costs by be eliminated with earlier diagnosis and treatment, because earlier diagnosis means possible prevention.

BioROSA has developed a biologically-based diagnostic for ASD. This is a one of a kind diagnostic, screening and diagnosisng by using machine learning and targeted metabolic analysis to process biological data into a reliable and accurate diagnosis. BioROSA’s technology is also being used in therapeutic trials to assess pharmacodynamics, patient stratification, and companion diagnostic, providing doctors with the tools they need to help enable earlier detection, diagnosis, and timely treatment. They are currently the highest accuracy solution on the market, at 95% sensitivity and 92% specificity.

BiomeSense

BiomeSense is developing an integrated biosensor & cloud-based bioinformatics platform to massively scale the collection and analysis of gut microbiome data, producing up to 30x the data for <10% of the cost compared to conventional methods. The enabling technology is a proprietary microfluidic biosensor that’s installed in the patient home, passively and continuously sampling the gut microbiome without any input from the patient.

The company’s first market is clinical research, exponentially increasing the data available during clinical trials. After this market, the company plans to rapidly move to clinical use as a companion / traditional diagnostic, and long-term, the ultimate vision is to become a widespread consumer product to enable everyone to track and understand their microbiome for better health.

BrickBuilt Therapeutics

Oral diseases are systemic diseases suffered by over 3.5 billion people worldwide: the periodontal disease suffered by half of American adults is linked to diseases such as heart disease, stroke, diabetes and obesity. BrickBuilt Therapeutics is developing a live biological product discovered by CEO Brian Klein. BrickBuilt is using this strain and others in its microbial library to develop microbiome-focused therapeutics for oral and systemic diseases.

This is not a single asset company: BrickBuilt scientists have built a platform to screen the world’s first purpose-built oral microbial culture collection to identify bacterial strains which have therapeutic merit.

Biomage

Single cell RNA-seq can be used to look at major genetic pathways across multiple cell types (e.g. different types of neurons or blood cells). Its many uses include searching for off-target effects of the lead candidate drug or identifying heterogeneous subclones within a tumour to create predictive models of response to different chemotherapeutics.

Biomage’s product, Cellscope, allows biologists with no prior bioinformatics training to analyse large, multi-sample single cell RNA-seq datasets, perform data integration and execute differential analysis of specific pathways.

BioFeyn

Making eating healthy fish sustainable. BioFeyn’s goal is to produce the most sustainable and nutrient-packed farmed fish on the market by optimizing existing ingredients that are already proven to be safe and sustainable. We add value for farmers, feed suppliers, and the environment.

BioFeyn adapts nanotechnologies based on human medicine to deliver nutrients and natural disease preventatives to fish. BioFeyn is pioneering a targeted, modular, delivery mechanism (what we call a “Feyn”) for compounds and nutrients that can be adapted to meet the most critical needs in the industry.

Beemmunity

One-third of food is pollinator-dependent, which means simply, pollinator survival represents human survival. The continuous use of pesticides in Industrialagriculture is decimating managed and native pollinator populations at an accelerating rate.

Beemmunity’s microsponge technology absorbs and neutralizes all pesticides when consumed by pollinators, improving survival rates from 0% to 80%. Beemmunity reduces the heavy burden placed on commercial beekeepers to meet the increasing demand for pollination services each season by delivering a product that treats pesticide exposure, reducing the number of hive losses and creating healthier hives.

Avisa Myko

Melanin is the pigment that tans our skin and makes our hair dark, but new research keeps coming out about melanin as a supermaterial and its wide variety of applications: blocking ionizing gamma radiation, acting as organic semiconductors for implantable electronics, and even generating electricity as a solar fuel cell and storing it in rechargable batteries. The primary problem is that melanin is too expensive to make these Industrialapplications scalable.

Avisa Myko is able to produce melanin from a proprietary strain of fungus. Whereas every is able to make it at $400/gram, they can make it at $0.20 per gram. They’ve validated their process at 50L scale while at IndieBio and they’re using the radiation blocking properties of melanin to formulate a suncreen, to replace the sunscreens that are toxic for the marine ecosystem. They are currently looking for partners for turning their melanin into dyes and radiation shielding (think: space).

Avalo

Half of all plant species are at risk from climate change

The world is changing faster than crops can adapt. To make crops more adaptive, we need a speedier way to alter their gene expression. Most plant genes are unexplored because their role is too subtle to be picked up by GWAS. Avalo.ai are leaders in interpretable machine learning in the realm of agricultural genetics.

This drastically narrows (by around 100x) the scope of candidate genes to validate. Paired with a system to deliver gene regulators via the soil, Avalo.ai attempts to replace the trial-and-error breeding of the past with a computational and programmable responsive system.

Allied Microbiota

Allied Microbiota develops microbes to degrade environmental contaminants. Their technology can tackle the most recalcitrant organic pollutants and break them down into harmless byproducts. Allied Microbiota uses a proprietary microbial strain, ThermO+™, to eliminate polychlorobiphenyls (PCBs) and polyaromatic hydrocarbons (PAHs), prominent soil contaminants, and dioxins, a prominent water contaminant. Treating contaminated sites restores land value and promotes businesses to build on formerly unusable land.

Aluna Medical Diagnostics

Before Aluna, patients had no way of objectively tracking their Asthma to understand triggers, measure lung function, and predict Asthma attachs.

Aluna’s platform empowers patients and clinicians to create personal action plans for their Asthma with FDA-cleared combination of a digital spirometer, a healthtracking platform, and a physician remote monitoring portal. They are currently fulfilling orders from clinicians at UCSF and family practice across the US. By enabling telemedicine Aluna has shown clinicians can intervene quickly when patients scores decrease and prevent severe symptoms.

With 41M patients suffering from Asthma or COPD, and use for COVID lung health recovery monitoring, Aluna is poised to be the solution for respiratory monitoring and care.

AsimicA

Biofermentation is often done in batches, because the cells (bacteria, yeast, etc.) that produce the product of interest will eventually mutate and cause the entire batch to become unproductive. This is why batches have to restart, undergoing an idle period, which costs time and money in labor and lost productivity.

Asimica’s solution to this problem is to create microbial stem cells, a technology that constantly generates “young” and productive factory cells, which solves two problems: 1) it prolongs the lifespan of a batch, which means more productivity and less downtime, and 2) more productivity from the constant production of younger factory cells. This can increase the productivity of biofermentation 2-3X per batch.

Aja Labs

Around the developing world, women and girls as young as eight years old are paid just $11 to $25 for selling their hair to supply the hair extension marketplace. The only readily available alternative is plastic synthetic hair, made from petrochemicals, from which consumers often experience toxicity reactions. At worst, these plastics potentially trigger endocrine disruption. Despite all these issues, extensions and weaves are still a fast-growing $7 billion market.

Aja Labs aims to be the ethical and more sustainable choice in hair extensions and weaves. Aja Labs makes human-like hair from plants by utilizing proprietary fiber processing, biotechnology, and microparticle technology to upcycle waste crop matter into premium, healthy hair extension products. The team is engineering sustainable fibers using materials that are better for human and planetary health.

Advanced Microbubbles

The difficult thing about treating tumors is that drugs have trouble penetrating their microenvironment. Chemotherapy itself is already a tricky balance of killing the cancer before it kills the patient, so increasing the dosage is already a risky gamble. Fortunately, the solution lies in the very same microbubbles that come out of your faucet.

Advanced Microbubbles has a patented method to size-isolate microbubbles, which can then be conjugated to any cancer drug. Combined with the humble ultrasound, these drug-bubble conjugates can then be spatially focused on the tumor using a medical device that exists in every clinic. This results in much less toxicity and much more drug penetrating the tumor.

Agrisea

The world’s food demands are expected to almost double by 2050, at the same time when climate change is stealing our supply our arable land for crop production, and a dearth of freshwater for both human and agricultural uses. Agrisea confronts these challenges by expanding our imagination, to think beyond these resources as the only way to grow crops.

Agrisea removes land and freshwater as agricultural constraints, by helping crops that are normally grown on land to grow in the ocean. By removing land and freshwater as key contraints they can grow crops 20X cheaper than on land. The scientific insight came from mangroves and seagrasses that have genes that are highly efficient in managing salt. Agrisea’s first crop is rice, a crop that feeds over half the world’s population, but is also a notorious methane producer because of the soil bacteria. By growing rice in floating ocean paddies, they will be able to provide food security to the world while also offsetting CO2e emissions